The effects of morphine and nalorphine on excretion of hydroxysteroids (3 : 21- hydroxysteroids 17-ketosteroids and aldosterone have been examined in rats. Urine, specimens an overview of collection methods, collection devices, specimen handling and transportation. A highly sensitive quantification technique.
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LC-ESI-MS/MS of hydroxysteroids is described using fusaric acid ester as a proton-affinitive tag in a positive mode. .